Based on the data collected from 2008 to 2010 in Eucalyptus plantation of Gaoyao city, Guangdong Province, the annual variation, seasonal variation and daily variation of soil temperature before and after rotation were analyzed. The result showed that the soil temperatures of various layers had a significantly positive correlation with the surface temperature. The annual variation of soil temperature showed a unimodal distribution and the annual amplitude became smaller in deeper layer. The daily variation followed a sinusoid and the daily amplitude became smaller in deeper layer. The peak value appeared later with the increasing of soil depth. The annual amplitude of the soil temperature in 2009 after the rotation (13.58 ℃) was smaller than in 2008 before the rotation (15.37 ℃). The peak value time appeared 1 hour ahead and the daily amplitude increased.